LOCUS       40924_16820        7909 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector pEDMS1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Yamashiro D, Minouchi Y, Ashiuchi M.
  TITLE     Moonlighting Role of a Poly-{gamma}-Glutamate Synthetase Component 
            from Bacillus subtilis: Insight into Novel Extrachromosomal DNA 
            Maintenance
  JOURNAL   Appl. Environ. Microbiol. 77 (8), 2796-2798 (2011)
  PUBMED    21357437
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Ashiuchi M, Yamashiro D.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2010) Contact:Makoto Ashiuchi Kochi University, 
            Agricultural Science, Graduate School of Integrated Arts and 
            Sciences; Monobe 200, Nankoku, Kochi 783-8502, Japan
REFERENCE   3  (bases 1 to 7909)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7909)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2011"; volume: "77"; issue: "8"; pages:
            "2796-2798"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUL-2010) Contact:Makoto Ashiuchi Kochi University, Agricultural
            Science, Graduate School of Integrated Arts and Sciences; Monobe 
            200, Nankoku, Kochi 783-8502, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..204
                     /codon_start=1
                     /gene="edmS"
                     /gene_synonym="pgsE"
                     /product="extra-chromosomal DNA-maintenance factor EdmS"
                     /label=edmS
                     /protein_id="BAK19406.1"
                     /translation="MKFVKAIWPFVAVAIVFMFMSAFKFNDQLTDQEKQKIDMEMNKIQ
                     QQEEPVNANK"
     gene            37..204
                     /gene="edmS"
                     /gene_synonym="pgsE"
                     /label=edmS
     rep_origin      complement(749..1337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1511..2368)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2369..2473)
                     /label=AmpR promoter
     CDS             complement(2640..4013)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(4216..5217)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     rep_origin      5368..5395
                     /label=Bacillus subtilis replication origin pBC16: oriU
                     /note="Bacillus subtilis replication origin pBC16: oriU"
     promoter        5642..5670
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 aatgacaaat ggtccaaact agtcggagtg ataaagatga aatttgtcaa agccatctgg
       61 ccgtttgttg ccgtagccat cgtgttcatg tttatgtcag cttttaaatt caatgatcag
      121 ctgacagatc aggaaaaaca gaagattgat atggaaatga ataaaatcca gcagcaggaa
      181 gaaccggtaa acgccaataa ataaggatcc tctagagtcg acctgcaggc atgcaagctt
      241 tcgcgagctc gagatctaga tatcgatgaa ttgatccgac gcgaggctgg atggccttcc
      301 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
      361 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
      421 gcctaacttc gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
      481 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
      541 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
      601 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
      661 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg
      721 gcgcatctcg ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt
      961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1801 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2101 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     2161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     2521 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattcc
     2581 tgttataaaa aaaggatcaa ttttgaactc tctcccaaag ttgatccctt aacgatttag
     2641 aaatcccttt gagaatgttt atatacattc aaggtaacca gccaactaat gacaatgatt
     2701 cctgaaaaaa gtaataacaa attactatac agataagttg actgatcaac ttccataggt
     2761 aacaaccttt gatcaagtaa gggtatggat aataaaccac ctacaattgc aatacctgtt
     2821 ccctctgata aaaagctggt aaagttaagc aaactcattc cagcaccagc ttcctgctgt
     2881 ttcaagctac ttgaaacaat tgttgatata actgttttgg tgaacgaaag cccacctaaa
     2941 acaaatacga ttataattgt catgaaccat gatgttgttt ctaaaagaaa ggaagcagtt
     3001 aaaaagctaa cagaaagaaa tgtaactccg atgtttaaca cgtataaagg acctcttcta
     3061 tcaacaagta tcccaccaat gtagccgaaa ataatgacac tcattgttcc agggaaaata
     3121 attacacttc cgatttcggc agtacttagc tggtgaacat ctttcatcat ataaggaacc
     3181 atagagacaa accctgctac tgttccaaat ataattcccc cacaaagaac tccaatcata
     3241 aaaggtatat ttttccctaa tccgggatca acaaaaggat ctgttacttt cctgatatgt
     3301 tttacaaata tcaggaatga cagcacgcta acgataagaa aagaaatgct atatgatgtt
     3361 gtaaacaaca taaaaaatac aatgcctaca gacattagta taattccttt gatatcaaaa
     3421 tgacctttta tccttacttc tttctttaat aatttcataa gaaacggaac agtgataatt
     3481 gttatcatag gaatgagtag aagataggac caatgaatat aatgggctat cattccacca
     3541 atcgctggac cgactccttc tcccatggct actatcgatc caataagacc aaatgcttta
     3601 cccctatttt cctttggaat atagcgcgca actacaacca ttacgagtgc tggaaatgca
     3661 gctgcaccag ccccttgaat aaaacgagcc ataataagta aggaaaagaa agaatggcca
     3721 acaaacccaa ttaccgaccc gaaacaattt attataattc caaataggag taaccttttg
     3781 atgcctaatt gatcagatag ctttccatat acagctgttc caatggaaaa ggttaacata
     3841 aaggctgtgt tcacccagtt tgtactcgca ggtggtttat taaaatcatt tgcaatatca
     3901 ggtaatgaga cgttcaaaac catttcattt aatacgctaa aaaaagataa aatgcaaagc
     3961 caaattaaaa tttggttgtg tcgtaaattc gattgtgaat aggatgtatt cacatttcac
     4021 cctccaataa tgagggcaga cgtagtttat agggttaatg atacgcttcc ctcttttaat
     4081 tgaaccctgt tacattcatt acacttcata attaattcct cctaaacttg attaaaacat
     4141 tttaccacat ataaactaag ttttaaattc agtatttcat cacttataca acaatatggc
     4201 ccgtttgttg aactactctt taataaaata atttttccgt tcccaattcc acattgcaat
     4261 aatagaaaat ccatcttcat cggctttttc gtcatcatct gtatgaatca aatcgccttc
     4321 ttctgtgtca tcaaggttta attttttatg tatttctttt aacaaaccac cataggagat
     4381 taacctttta cggtgtaaac cttcctccaa atcagacaaa cgtttcaaat tcttttcttc
     4441 atcatcggtc ataaaatccg tatcctttac aggatatttt gcagtttcgt caattgccga
     4501 ttgtatatcc gatttatatt tatttttcgg tcgaatcatt tgaactttta catttggatc
     4561 atagtctaat ttcattgcct ttttccaaaa ttgaatccat tgtttttgat tcacgtagtt
     4621 ttctgtattc ttaaaataag ttggttccac acataccaat acatgcatgt gctgattata
     4681 agaattatct ttattattta ttgtcacttc cgttgcacgc ataaaaccaa caagattttt
     4741 attaattttt ttatattgca tcattcggcg aaatccttga gccatatctg acaaactctt
     4801 atttaattct tcgccatcat aaacattttt aactgttaat gtgagaaaca accaacgaac
     4861 tgttggcttt tgtttaataa cttcagcaac aaccttttgt gactgaatgc catgtttcat
     4921 tgctctcctc cagttgcaca ttggacaaag cctggattta caaaaccaca ctcgatacaa
     4981 ctttctttcg cctgtttcac gattttgttt atactctaat atttcagcac aatcttttac
     5041 tctttcagcc tttttaaatt caagaatatg cagaagttca aagtaatcaa cattagcgat
     5101 tttcttttct ctccatggtc tcacttttcc actttttgtc ttgtccacta aaacccttga
     5161 tttttcatct gaataaatgc tactattagg acacataata ttaaaagaaa cccccatcta
     5221 tttagttatt tgtttggtca cttataactt taacagatgg ggtttttctg tgcaaccaat
     5281 tttaagggtt ttcaatactt taaaacacat acataccaac acttcaacgc acctttcagc
     5341 aactaaaata aaaatgacgt tatttctata tgtatcaaga taagaaagaa caagttcaaa
     5401 accatcaaaa aaagacacct tttcaggtgc tttttttatt ttataaactc attccctgat
     5461 ctcgacttcg ttcttttttt acctctcggt tatgagttag ttcaaattcg ttctttttag
     5521 gttctaaatc gtgtttttct tggaattgtg ctgttttatc ctttaccttg tctacaaacc
     5581 ccttaaaaac gtttttaaag gcttttaagc cgtctgtacg ttccttaagg aattctcatg
     5641 tttgacagct tatcatcgat aagctttaat gcggtagttt atcacagtta aattgctaac
     5701 gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca
     5761 ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata
     5821 tcgtccattc cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga
     5881 tgcaatttct atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag
     5941 tcctgctcgc ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg
     6001 tcctgtggat ctgacgcgtg taactgcgaa ggtaaaacgg gtgattgaaa ataaactaac
     6061 aaatgaagag gaacgaaaga gaagtctaga atttgttacg tttatatcgg atgaattact
     6121 gcaaaatgat acggagcctc gcacatttat tattcagaac tctcttttat cgctagagaa
     6181 aatccatact ctcgcacaga ccaaagaggt agaggaatat aaagaagtca taactactct
     6241 gacaagacat gattcataat ttgaaaagca ggtaaactaa cccaaaaggc aagactctcc
     6301 gtagtttaga aaagagcagg ttgctaataa catataaaca gccagttgcc gttatgatag
     6361 gtgactggct gcatgggatg aaaaggtgag ggtggagaca gacataacac tcttaataga
     6421 agagggtaat tctttctctt ttatagaaaa tcaattaatt gaaagtagct ccttcattct
     6481 taagatcaac gtgatatagg tttgctaacc tttgcgttca cttaactaac ttataggggt
     6541 aacacttaaa aaagaatcaa taacgataga aaccgctcct aaagcaggtg cattttttcc
     6601 taacgaagaa ggcaatagtt cacatttatt gtctaaatga gaatggactc tagaagaaac
     6661 ttcgttttta atcgtattta aaacaatggg atgagattca attatatgat ttctcaagat
     6721 aacagcttct atatcaaatg tattaaggat attggttaat ccaattccga tataaaagcc
     6781 aaagttttga agtgcattta acatttctac atcattttta tttgcgcgtt ccacaatctc
     6841 ttttcgagaa atattctttt cttctttaga gagcgaagcc agtaacgctt tttcagaagc
     6901 atataattcc caacagcctc gatttccaca gctgcatttg ggtccattaa aatctatcgt
     6961 catatgaccc atttccccag aaaaaccctg aacaccttta tacaattcgt tgttaataac
     7021 aagtccagtt ccaattccga tattaatact gatgtaaacg atgttttcat agttttttgt
     7081 cataccaaat actttttcac cgtatgctcc tgcattagct tcattttcaa caaaaaccgg
     7141 aacattaaac tcactctcaa ttaaaaactg caaatctttg atattccaat ttaagttagg
     7201 catgaaaata atttgctgat gacgatctac aaggcctgga acacaaattc ctattccgac
     7261 tagaccataa ggggactcag gcatatgggt tacaaaacca tgaataagtg caaataaaat
     7321 ctcttttact tcactagcgg aagaactaga caagtcagaa gtcttctcga gaataatatt
     7381 tccttctaag tcggttagaa ttccgttaag atagtcgact cctatatcaa taccaatcga
     7441 gtagcctgca ttcttattaa aaacaagcat tacaggtctt ctgccgcctc tagattgccc
     7501 tgccccaatt tcaaaaataa aatctttttc aagcagtgta tttacttgag aggagacagt
     7561 agacttgttt aatcctgtaa tctcagagag agttgccctg gagacagggg agttcttcaa
     7621 aatttcatct aatattaatt tttgattcat tttttttact aaagcttgat ctgcaatttg
     7681 aataataacc actcctttgt ttatccaccg aactaagttg gtgttttttg aagcttgaat
     7741 tagatattta aaagtatcat atctaatatt ataactaaat tttctaaaaa aaacattgaa
     7801 ataaacattt attttgtata tgatgagata aagttagttt attggataaa caaactaact
     7861 caattaagat agttgatgga taaacttgtt cacttaaatc aaaggggga
//
